GENOMIC ARCHITECTURE OF SEASONAL ADAPTATION DURING SPECIATION


 

Seasonality appears to be a major driver of speciation, particularly in plant-eating insects that specialize on hosts that are only available during limited seasonal windows. The apple maggot fly,  Rhagoletis pomonella, provides a textbook example, historically feeding on native hawthorns in North America but now shifting to exploit apples, an introduced crop. Apples fruit earlier, the flies have only one generation per year, and the apple host race has evolved earlier seasonal timing via changes in pupal diapause regulation.

 

RhagoLifeCycle

Rhagoletis pomonella life cycle. Diagram From Dambroski HR and JL Feder. J. Evol. Biol. 20 2101–2112. doi:10.1111/j.1420-9101.2007.01435.x

 

This separation in time is driving ecological speciation among the host races. Further, the entire Rhagoletisspecies complex appears to be an adaptive radiation with highly genetically similar species that differ markedly in seasonal timing, suggesting that this is a major mechanism for speciation. A number of studies have now shown that ecological speciation can drive genetic divergence across the genome, including in R. pomonella. Our research attempts to characterize the physiological and genetic targets of divergent selection, and to determine how this genetic architecture shapes genome-wide divergence

 

Divergence

Regions of high genomic divergence correspond with regions that respond to selection on seasonality (Only Chr. I,II,III shown). From Egan et al. 2015, Ecol. Letters.

 

We have found a striking correspondence between genomic divergence between host races and genomic regions that respond to artificial selection on seasonality. The specific targets of selection appear to be several developmental regulatory events related to diapause overwintering and the timing of adult emergence. We are applying studies of developmental series of endocrine sensitivity and gene expression in combination with Genome Wide Association Studies (GWAS) to disentangle these targets of selection and determine whether they evolve as constrained or independent modules.

pupa

Read more about diapause regulation here